Navigation
Personal tools


 
You are here: Home → Core Facilities → Proteomics → Instruments, Methods and Applications
Document Actions

Instruments, Methods and Applications

 

            Instrumente, Methoden und Anwendungen 

  

  Instruments

 

MS

        Agilent 6340 ETD Ion Trap
        Agilent 6460 Triple Quadrupole
        Agilent 6520 Q-TOF

        all Agilent MS are equipped with a nanoChip HPLC system

 MS
         Thermo LTQ-Orbitrap XL
         with online nanoHPLC (Agilent 1200 Series)

 HPLC

          Agilent 1200 Series with fraction collector
          Dionex Ultimate 3000 (Titanium) with Probot fraction collector

Elektrophoresis

         Agilent OFFGEL elektrophoresis
         ETC horizontale gel elektrophoresis (Flat Top)                                     
         BioRad 1D gel electrophoresis
         Invitrogen 1D gel electrophoresis

 Elektrophorese

 Laser-Scanner        GE Typhoon 9400

Software   

         Mascot Server
         Mascot Distiller (Quantitation Toolbox)                                                   
         Mascot Integra
         Scaffold
         PEAKS
         MaxQuant

 Software

 

 

  Methoden

 

  nanoLC-MS/MS

  Enrichment of phosphopeptides with TiO2 (Ultimate 3000)

  Electron Transfer Dissociation (ETD)

  Multiple Reaction Monitoring (MRM)

  Metabolic Labeling (15N und SILAC)

  

  Anwendungen

 

  Protein ID

  De novo Sequenzierung

  Analysis of posttranslational modifications (PTM)                                    

  Molecular mass determination of intact proteins

  ID of protein interacting partners

  Quantitative analysis

 


by Daniel Bichsel — last modified 31.03.2010 12:12