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Methods and Instrumentation
GC-MS Metabolite Profiling in plants, animals and microorganisms
As a routine method we are profiling relative concentration changes in primary metabolites (sugars, sugar-alcohols, amino acids, organic acids and phosphorylated compounds). The mainly polar metabolites are extracted from the tissue, derivatised and the derivates are quantified and identified with an Agilent GC-Quadrupole-MS System, which can operate in EI and CI mode.
Several more targeted methods for analysing particular (also non-polar) compounds are developed in cooperation with the various users.
Fig.1: Example Chromatogram for a GC-MS primary metabolite profiling in Physcomitrella patens
Fig.2: Example for a cluster analysis for the relative changes of metabolite concentrations in Ricinus communis under anoxia.