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05.05.2010 Hausseminar: Ursula Schäffer, AG Schmidt


erstellt von Arkady Emelyanov zuletzt verändert: 04.05.2010 14:28
Was Vortrag Lecture Seminar
Wann 05.05.2010
von 10:15 bis 11:00
Wo Habsburgerstrasse 49, 79104 Freiburg
Name Ursula Schäffer
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Establishment of tightly controllable expression systems for the model organism C. elegans

 

The nematode C. elegans is a well investigated model organism with many advantages. Unfortunatly, until now no well controllable gene expression systems are available.

To solve this issue, the Tetracycline activatable Tet-on expression system was adapted for the use in C. elegans to allow both spatially and temporally controlled gene expression at the same time. Furthermore, the Tet-on system will be combined with the SnAvi-tag – a tandem tag for protein purification. This combination should allow to specifically purify a protein of interest with its complex partners from a specific tissue of C. elegans at defined time points.