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Instruments, Methods and Applications

 

Instruments, Methods and Applications

 

Instruments

 

MS

  • Agilent 6340 ETD Ion Trap
  • Agilent 6460 Triple Quadrupole
  • Agilent 6520 Q-TOF

all Agilent MS are equipped with a nanoChip HPLC system

 MS
  • Thermo LTQ-Orbitrap XL

with online nanoHPLC (Agilent 1200 Series)

 

HPLC

  • Agilent 1200 Series with fraction collector
  • Dionex Ultimate 3000 (Titanium) with Probot fraction collector

 

Elektrophoresis

  • Agilent OFFGEL elektrophoresis
  • ETC horizontale gel elektrophoresis (Flat Top)
  • BioRad 1D gel electrophoresis
  • Invitrogen 1D gel electrophoresis
 Electrophoresis

 

Laser-Scanner

  • GE Typhoon 9400

 

Software

  • Mascot Server
  • Mascot Distiller (Quantitation Toolbox)
  • Mascot Integra
  • Scaffold
  • PEAKS
  • MaxQuant
 Software

 

Methods

  • nanoLC-MS/MS
  • Enrichment of phosphopeptides with TiO2 (Ultimate 3000)
  • Electron Transfer Dissociation (ETD)
  • Multiple Reaction Monitoring (MRM)
  • Metabolic Labeling (15N und SILAC)

 

Applications

  • Protein ID
  • De novo Sequenzierung
  • Analysis of posttranslational modifications (PTM)
  • Molecular mass determination of intact proteins
  • ID of protein interacting partners
  • Quantitative analysis